Mr Rees-Mogg questioned Mrs May during PMQs to establish whether Britain was going back on the red lines set out in the Prime Minister’s Florence speech.
Ms Villiers told Channel 4 News she doesn’t agree with her political colleague and that the Prime Minister has always been very clear.
She said: “I think the Prime Minister continues to be clear about her red lines.
“We will be leaving the customs union and the single market but we are going to work towards a comprehensive trade deal.”
The MP for Chipping Barnet said that the Prime Minister’s position has been the same since soon after the June 2016 referendum.
Ms Villiers said: “That’s the position now as it always has been since the days fairly shortly after the Referendum.”
Mr Rees-Mogg later told Nigel Farage he thinks both the Chancellor and the Prime Minister are wrong in their approach towards Brexit.
He said: “I cannot understand why a Chancellor, when money is so short, when we have the threat to the Royal Marines, when the newspapers have been saying the army may go down to 50,000, when the NHS needs money, that he should be saying ‘well, we’ll give the £40billion because we committed to in a previous life.
“I thought that was a very deeply disappointing comment.”
Mr Rees-Mogg also gave his ultimate Brexit red line and said the PM should concentrate on securing an agreement on the European Court of Justice.
He said: “My reddest of red lines is always the European Court of Justice because it seems to me as long as we remain under the European Court of Justice, we haven’t left the European Union.”
On Wednesday a poll by Conservative Home revealed the Brexiteer is seen as a better choice than Michael Gove, David Davis and Boris Johnson as the next Tory leader.